| Gene name |
SPBP18G5.03 |
| Gene ID |
50/F01 |
| Gene synonyms/obsolete |
|
| Gene product |
hypothetical protein;
sequence orphan |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1335 |
| ORF length (spliced) |
1293 |
| Entry clone length |
1335 |
| No. of intron |
1 |
| Sequence status |
Partially
sequenced |
| Sequence results |
#Low sequence
quality |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBP18G5.03.Fd |
| Fwd primer SEQ |
GGGGACAAGTTTGTACAAAAAAGCAGGCTCTCATATGCAATTGCAATCGCCATT |
| Rev primer name |
SPBP18G5.03.Rv |
| Rev primer SEQ |
GGGGACCACTTTGTACAAGAAAGCTGGGTAATTTAAACTCTTCCAACCT |
| Amino acid length |
430 |
| Molecular weight |
48.7 |
| Isoelectric point (calc.) |
4.8 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
cytosol |
| Comments for localization |
cytoplasmic dots by
over expression |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |