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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC977.02
Gene ID 51/B03
Gene synonyms/obsolete
Gene product telomeric duplication; possibly Sp specific
Entry clone Cloned#
ORF length (unspliced) 441
ORF length (spliced)
Entry clone length 441
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAC977.02.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGCATTGTTAAAAAAAAT
Rev primer name SPAC977.02.Rv
Rev primer SEQ AGAAAGCTGGGTATCGTGATATGAATCTTGAT
Amino acid length 146
Molecular weight 17.1
Isoelectric point (calc.) 10.5
Signal SEQ
No. of transmembrane domain 1
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus; cytoplasmic dots; nuclear envelope?
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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