Gene name |
SPBC1348.04 |
Gene ID |
51/B02 |
Gene synonyms/obsolete |
SPAC1348.04 |
Gene product |
sterol
methyltransferase; duplicated in Sp; similar to Sp SPAC750.03C
and SPAC977.03; no apparent Sc ortholog |
Entry clone |
Cloned |
ORF length (unspliced) |
438 |
ORF length (spliced) |
|
Entry clone length |
438 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPAC1348.04.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAATCTTGTACAACTGGG |
Rev primer name |
SPAC1348.04.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATAAAGAAAGCTTTTTGACA |
Amino acid length |
145 |
Molecular weight |
16.5 |
Isoelectric point (calc.) |
9.8 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
cytosol=nucleus |
Comments for localization |
too dark to
photo |
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Zeiss |