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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC22A12.04c
Gene ID 51/B01
Gene synonyms/obsolete rps2202; rps15a-1; rps22-1
Gene product 40S ribosomal protein S15A/S22A
Entry clone Cloned
ORF length (unspliced) 393
ORF length (spliced)
Entry clone length 393
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAC22A12.04.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGTTCGTCAATCTGTTCT
Rev primer name SPAC22A12.04.Rv
Rev primer SEQ AGAAAGCTGGGTAATAGAAGAAACCAAGAATC
Amino acid length 130
Molecular weight 14.7
Isoelectric point (calc.) 10.9
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus>>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Zeiss

Image information
YFP 2 images) See all images

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