Chemical Genetics Laboratory
PREVIOUS  NEXT
Schizosaccharomyces pombe Postgenome Database

Gene name SPAC212.08c
Gene ID 51/C03
Gene synonyms/obsolete
Gene product Sp specific families; 66294 domain; possibly Sp specific; telomeric duplication
Entry clone Cloned
ORF length (unspliced) 837
ORF length (spliced)
Entry clone length 837
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAC212.08.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCCCCTTTAATAGTTGG
Rev primer name SPAC212.08.Rv
Rev primer SEQ AGAAAGCTGGGTAGCTACCGTTAAATGTCTGC
Amino acid length 278
Molecular weight 31
Isoelectric point (calc.) 5.5
Signal SEQ Predicted (N-terminus)
No. of transmembrane domain 1
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) ER
Comments for localization too dark to photo
Effect of LMB on protein localization no change
Microscope used for observation Zeiss

Image information
YFP 4 images) See all images

For plasmid request Click!
Copyright (c) RIKEN (The Institute of Physical and Chemical Research), Japan. All rights reserved.