| Gene name |
SPCC1919.06c |
| Gene ID |
51/C11 |
| Gene synonyms/obsolete |
wtf25;
wtf9-pseudo |
| Gene product |
wtf element |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1065 |
| ORF length (spliced) |
750 |
| Entry clone length |
1065 |
| No. of intron |
4 |
| Sequence status |
Finished |
| Sequence results |
14A:G /
942A:addition |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPCC1183.10.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAAGAATAATTGCACTTC |
| Rev primer name |
SPCC1183.10.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAGGCTTCACTTTCAGGGTTC |
| Amino acid length |
249 |
| Molecular weight |
28.4 |
| Isoelectric point (calc.) |
5.2 |
| Signal SEQ |
|
| No. of transmembrane domain |
3 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
nuclear envelope |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |