Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPBPB21E7.07
Gene ID 52/A08
Gene synonyms/obsolete aes1; SPAPB21E7.07
Gene product enhancer of RNA-mediated gene silencing; phrenazine biosynthesis-like protein
Entry clone Cloned
ORF length (unspliced) 891
ORF length (spliced)
Entry clone length 891
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAPB21E7.07.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGACTGAACACTCATTTAA
Rev primer name SPAPB21E7.07.Rv
Rev primer SEQ AGAAAGCTGGGTAAATAAACGATTTTCCAGAA
Amino acid length 296
Molecular weight 32.2
Isoelectric point (calc.) 5.1
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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