Gene name |
SPCP31B10.08c |
Gene ID |
05/B02 |
Gene synonyms/obsolete |
rpl35a; rpl33 |
Gene product |
60S ribosomal protein
L35a |
Entry clone |
Cloned |
ORF length (unspliced) |
517 |
ORF length (spliced) |
327 |
Entry clone length |
517 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
(-8)G:addition /
19A:T |
Comments |
5' terminus is
frameshifted. |
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPCP31B10.08.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCCAGCTCAAGGACATAG |
Rev primer name |
SPCP31B10.08.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAATGTTGGAGGGGTAAAGC |
Amino acid length |
108 |
Molecular weight |
12.1 |
Isoelectric point (calc.) |
11.1 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
no apparent
signal |
Comments for localization |
|
Effect of LMB on protein
localization |
not determined |
Microscope used for
observation |
Leica |