| Gene name |
SPAC21E11.04 |
| Gene ID |
07/B11 |
| Gene synonyms/obsolete |
ppr1 |
| Gene product |
experimentally
characterised; L-azetidine-2-carboxylic acid
acetyltransferase; GNAT family; detoxifies a proline analogue;
no apparent Sc ortholog |
| Entry clone |
Cloned# |
| ORF length (unspliced) |
630 |
| ORF length (spliced) |
|
| Entry clone length |
630 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPAC21E11.04.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAAGGATCCAAATACTAT |
| Rev primer name |
SPAC21E11.04.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATTTTAAGGCATCTTCAAGT |
| Amino acid length |
209 |
| Molecular weight |
23.8 |
| Isoelectric point (calc.) |
8.3 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
cytosol=nucleus |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |