| Gene name |
SPAC1250.03 |
| Gene ID |
07/B12 |
| Gene synonyms/obsolete |
ubc14 |
| Gene product |
ubiquitin conjugating
enzyme; involved in ubiquitin conjugation |
| Entry clone |
Cloned |
| ORF length (unspliced) |
630 |
| ORF length (spliced) |
468 |
| Entry clone length |
630 |
| No. of intron |
2 |
| Sequence status |
Finished |
| Sequence results |
315G:A |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC1250.03.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCGTCTGCATCACCTTC |
| Rev primer name |
SPAC1250.03.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAGACTTGGCGAATTGCTCG |
| Amino acid length |
155 |
| Molecular weight |
17.7 |
| Isoelectric point (calc.) |
6.5 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LRSVLEQILQL |
| Localization (YFP) |
no apparent
signal |
| Comments for localization |
|
| Effect of LMB on protein
localization |
not determined |
| Microscope used for
observation |
Zeiss |