Gene name |
SPBC11G11.06c |
Gene ID |
07/C01 |
Gene synonyms/obsolete |
sme1 |
Gene product |
small nuclear
ribonucleoprotein E; involved in mRNA splicing |
Entry clone |
Cloned |
ORF length (unspliced) |
630 |
ORF length (spliced) |
255 |
Entry clone length |
630 |
No. of intron |
3 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC11G11.06.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCAGGACGCGTGCAAAA |
Rev primer name |
SPBC11G11.06.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAGATTGCTTGAATTAGTGTA |
Amino acid length |
84 |
Molecular weight |
9.6 |
Isoelectric point (calc.) |
9.3 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nucleus>>cytosol
|
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Zeiss |