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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC1604.09c
Gene ID 11/D02
Gene synonyms/obsolete
Gene product exonuclease
Entry clone Cloned
ORF length (unspliced) 857
ORF length (spliced) 783
Entry clone length 857
No. of intron 1
Sequence status Finished
Sequence results (-1)T:A
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC1604.09.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCTTCTTCCAATTGGGA
Rev primer name SPBC1604.09.Rv
Rev primer SEQ AGAAAGCTGGGTATGACTTTGACAAACCTTTG
Amino acid length 260
Molecular weight 29.6
Isoelectric point (calc.) 10.3
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleolus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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