Gene name |
SPBC1604.09c |
Gene ID |
11/D02 |
Gene synonyms/obsolete |
|
Gene product |
exonuclease |
Entry clone |
Cloned |
ORF length (unspliced) |
857 |
ORF length (spliced) |
783 |
Entry clone length |
857 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
(-1)T:A |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC1604.09.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCTTCTTCCAATTGGGA |
Rev primer name |
SPBC1604.09.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATGACTTTGACAAACCTTTG |
Amino acid length |
260 |
Molecular weight |
29.6 |
Isoelectric point (calc.) |
10.3 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nucleolus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |