Gene name |
SPBC1604.04 |
Gene ID |
14/D11 |
Gene synonyms/obsolete |
|
Gene product |
MC transporter;
carrier for the cofactor for thiamine pyrophosphate |
Entry clone |
Cloned |
ORF length (unspliced) |
991 |
ORF length (spliced) |
945 |
Entry clone length |
991 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC1604.04.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGGCCTTGCAAAGCAAAA |
Rev primer name |
SPBC1604.04.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAGCTTCCGGAGAGGACAGT |
Amino acid length |
314 |
Molecular weight |
34.9 |
Isoelectric point (calc.) |
9.7 |
Signal SEQ |
|
No. of transmembrane domain |
6 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
mitochondrion |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |