| Gene name |
SPBC1D7.03c |
| Gene ID |
21/D11 |
| Gene synonyms/obsolete |
SPBC1D7.03 |
| Gene product |
cyclin-like
protein |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1386 |
| ORF length (spliced) |
|
| Entry clone length |
1386 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
727A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC1D7.03.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCGTTTCCTTATCAGCA |
| Rev primer name |
SPBC1D7.03.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAGTCATTGCATAGCGATTG |
| Amino acid length |
461 |
| Molecular weight |
50.6 |
| Isoelectric point (calc.) |
8.3 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
nuclear dots |
| Comments for localization |
a lot of moving
cytoplasmic dots by over expression |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |