Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC17G8.13c
Gene ID 21/E01
Gene synonyms/obsolete mst2
Gene product MYST-family HAT (pers. comm. S. Forsburg); histone acetyltransferase; MOZ/SAS domain; zinc finger protein; zf-C2H2 type (inferred from context); involved in transcriptional silencing
Entry clone Cloned
ORF length (unspliced) 1387
ORF length (spliced) 1224
Entry clone length 1387
No. of intron 2
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC17G8.13.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCCGGCGACTATTTTGAA
Rev primer name SPAC17G8.13.Rv
Rev primer SEQ AGAAAGCTGGGTAAACGGAATCCAGATGATGA
Amino acid length 407
Molecular weight 47.2
Isoelectric point (calc.) 9
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus; cytosol with filamentous structure
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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