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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC6F6.04c
Gene ID 23/F08
Gene synonyms/obsolete
Gene product membrane transporter; no apparent Sc ortholog
Entry clone Cloned
ORF length (unspliced) 1470
ORF length (spliced)
Entry clone length 1470
No. of intron 0
Sequence status Finished
Sequence results 402A:G / 960T:C / 1017A:G / 1292A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC6F6.04.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGTTGCTCTAGTCAATAA
Rev primer name SPAC6F6.04.Rv
Rev primer SEQ AGAAAGCTGGGTAAACGTAGACCTGGTCGCTT
Amino acid length 489
Molecular weight 53.3
Isoelectric point (calc.) 7.8
Signal SEQ
No. of transmembrane domain 10
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) ambiguous structure
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

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