Gene name |
SPAC6F6.04c |
Gene ID |
23/F08 |
Gene synonyms/obsolete |
|
Gene product |
membrane transporter;
no apparent Sc ortholog |
Entry clone |
Cloned |
ORF length (unspliced) |
1470 |
ORF length (spliced) |
|
Entry clone length |
1470 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
402A:G / 960T:C /
1017A:G / 1292A:G |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC6F6.04.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGTTGCTCTAGTCAATAA |
Rev primer name |
SPAC6F6.04.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAACGTAGACCTGGTCGCTT |
Amino acid length |
489 |
Molecular weight |
53.3 |
Isoelectric point (calc.) |
7.8 |
Signal SEQ |
|
No. of transmembrane domain |
10 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
ambiguous
structure |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |