Chemical Genetics Laboratory
PREVIOUS  NEXT
Schizosaccharomyces pombe Postgenome Database

Gene name SPAC22H10.08
Gene ID 24/H05
Gene synonyms/obsolete
Gene product hypothetical protein; similar to Sp SPCC16A11.03C
Entry clone Cloned
ORF length (unspliced) 1535
ORF length (spliced) 1479
Entry clone length 1535
No. of intron 1
Sequence status Finished
Sequence results 728T:C / 1279A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC22H10.08.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGACGCTGAAGCCTTAAG
Rev primer name SPAC22H10.08.Rv
Rev primer SEQ AGAAAGCTGGGTACTCACCATTGAAGCTAAGA
Amino acid length 492
Molecular weight 56.5
Isoelectric point (calc.) 6.3
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LWALSDLEL
Localization (YFP) nucleus>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 1 images) See all images

For plasmid request Click!
Copyright (c) RIKEN (The Institute of Physical and Chemical Research), Japan. All rights reserved.