Gene name |
SPAC23C11.06c |
Gene ID |
25/G08 |
Gene synonyms/obsolete |
|
Gene product |
conserved
hypothetical; hypothetical protein |
Entry clone |
Cloned |
ORF length (unspliced) |
1608 |
ORF length (spliced) |
|
Entry clone length |
1608 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
320C:T |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC23C11.06.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAATACTTCTCAAGACGT |
Rev primer name |
SPAC23C11.06.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAATTGGGCCACCCACCGTAT |
Amino acid length |
535 |
Molecular weight |
60.1 |
Isoelectric point (calc.) |
7 |
Signal SEQ |
Predicted
(N-terminus) |
No. of transmembrane domain |
5 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LLITIICHLFL/LWAFLMSLWI/LSVILTILTI |
Localization (YFP) |
cytosol |
Comments for localization |
bright dots by over
expression |
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |