| Gene name |
SPBC354.02c |
| Gene ID |
26/E09 |
| Gene synonyms/obsolete |
sec61 |
| Gene product |
translocon (alpha
subunit); involved in protein-ER targeting; essential |
| Entry clone |
Cloned# |
| ORF length (unspliced) |
1896 |
| ORF length (spliced) |
1440 |
| Entry clone length |
1896 |
| No. of intron |
5 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPBC354.02.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAGTGATTGTAAGACCAT |
| Rev primer name |
SPBC354.02.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATTGCATCATGGGAGTAACG |
| Amino acid length |
479 |
| Molecular weight |
53.2 |
| Isoelectric point (calc.) |
8.9 |
| Signal SEQ |
Predicted
(N-terminus) |
| No. of transmembrane domain |
10 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LGAGICLLLIL |
| Localization (YFP) |
ER; Golgi? |
| Comments for localization |
ER-Golgi? |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |