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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC1F3.10c
Gene ID 28/C03
Gene synonyms/obsolete
Gene product metallopeptidase activity; mitochondrial processing; peptidase family M3
Entry clone Cloned
ORF length (unspliced) 2289
ORF length (spliced)
Entry clone length 2289
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC1F3.10.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCAAGTAAGAACTTTGCT
Rev primer name SPAC1F3.10.Rv
Rev primer SEQ AGAAAGCTGGGTAAAAGGTGGACGTCGCTTTA
Amino acid length 762
Molecular weight 86.2
Isoelectric point (calc.) 8.1
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LKKILNNLAL
Localization (YFP) mitochondrion
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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