Gene name |
SPAC15A10.10 |
Gene ID |
28/D04 |
Gene synonyms/obsolete |
mde6 |
Gene product |
involved in
sporulation; involved in mitosis; Mei4p-dependent expression;
Muskelin homolog; kelch repeat protein; non-essential; no
apparent Sc ortholog |
Entry clone |
Cloned# |
ORF length (unspliced) |
2330 |
ORF length (spliced) |
2151 |
Entry clone length |
2330 |
No. of intron |
4 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPAC15A10.10.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGAACGTAAAGAATTGTC |
Rev primer name |
SPAC15A10.10.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAGTGAAAAATTCAATCATA |
Amino acid length |
716 |
Molecular weight |
83.6 |
Isoelectric point (calc.) |
7.5 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LSDFWKLDI/LSNVSLQLHL |
Localization (YFP) |
a few cytoplasmic
dots |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |