| Gene name |
SPBC13E7.01 |
| Gene ID |
29/B01 |
| Gene synonyms/obsolete |
cwf22;
SPBC15D4.16 |
| Gene product |
eukaryotic conserved
protein; MIF4G domain; MA3 domain; complexed with Cdc5p; human
ortholog fSAPb has been isolated with functional spliceosomes
|
| Entry clone |
Cloned#/Sequence
mismatch |
| ORF length (unspliced) |
2505 |
| ORF length (spliced) |
|
| Entry clone length |
2505 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
2345G:addition |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPBC13E7.01.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGAGAAAGAAGATAAATC |
| Rev primer name |
SPBC13E7.01.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAGTGGTATGTTTTTTGCTT |
| Amino acid length |
834 |
| Molecular weight |
96.3 |
| Isoelectric point (calc.) |
7.9 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
no apparent
signal |
| Comments for localization |
|
| Effect of LMB on protein
localization |
not determined |
| Microscope used for
observation |
Leica |