Gene name |
SPBC776.04 |
Gene ID |
29/D04 |
Gene synonyms/obsolete |
sec2302; sec23-b |
Gene product |
GTPase activator;
involved in intracellular protein transport; COPII-coated
vesicle component; similar to Sp SPCC31H12.07 (paralog) |
Entry clone |
Cloned |
ORF length (unspliced) |
2557 |
ORF length (spliced) |
2298 |
Entry clone length |
2557 |
No. of intron |
3 |
Sequence status |
Finished |
Sequence results |
124A:G / 1231C:T |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC776.04.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAATTTTGAGGACATAGA |
Rev primer name |
SPBC776.04.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAGATATGACAGCCATTTTA |
Amino acid length |
765 |
Molecular weight |
85.3 |
Isoelectric point (calc.) |
6.7 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LKDAVIVSLSL |
Localization (YFP) |
cytosol=nucleus |
Comments for localization |
cytoplasmic dots by
over expression |
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |