Gene name |
SPAC222.14c |
Gene ID |
29/D05 |
Gene synonyms/obsolete |
|
Gene product |
conserved
hypothetical; GTP-binding protein; similarity in the
C-terminal region to human GBP1, an interferon-induced
GTP-binding protein |
Entry clone |
Cloned# |
ORF length (unspliced) |
2567 |
ORF length (spliced) |
2289 |
Entry clone length |
2567 |
No. of intron |
3 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPAC222.14.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAGTACTGCTTCCAATAG |
Rev primer name |
SPAC222.14.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATGAAAGCGCAGTTTTTTCT |
Amino acid length |
762 |
Molecular weight |
87.5 |
Isoelectric point (calc.) |
4.8 |
Signal SEQ |
|
No. of transmembrane domain |
1 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LKTVLEVNLQL |
Localization (YFP) |
cytosol; cytoplasmic
dots |
Comments for localization |
|
Effect of LMB on protein
localization |
changed to: nuclear
dots; nucleus |
Microscope used for
observation |
DeltaVision |