| Gene name |
SPAC24B11.12c |
| Gene ID |
30/G07 |
| Gene synonyms/obsolete |
|
| Gene product |
P-type ATPase;
aminophospholipid translocase |
| Entry clone |
Cloned |
| ORF length (unspliced) |
4288 |
| ORF length (spliced) |
4209 |
| Entry clone length |
4288 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
125A:G / 1464T:C /
1695A:G / 1878C:T / 2640A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC24B11.12.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGAAAGTGTTGAGGAAAA |
| Rev primer name |
SPAC24B11.12.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAGGCCGTAACGGCTGTTCA |
| Amino acid length |
1402 |
| Molecular weight |
159.3 |
| Isoelectric point (calc.) |
5.2 |
| Signal SEQ |
|
| No. of transmembrane domain |
10 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
210 |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LPNFKALRL/LPRTLYFGLPL/LEGLRTLCI |
| Localization (YFP) |
ER |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |