| Gene name |
SPBC119.13c |
| Gene ID |
33/B09 |
| Gene synonyms/obsolete |
prp31 |
| Gene product |
involved in mRNA
splicing; links splicing and meiosis; essential |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1557 |
| ORF length (spliced) |
|
| Entry clone length |
1557 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
290A:G / 623T:C /
885T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC119.13.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCACTGGCAGATGAATT |
| Rev primer name |
SPBC119.13.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATGAAAATTTATTTTTCGGC |
| Amino acid length |
518 |
| Molecular weight |
57.7 |
| Isoelectric point (calc.) |
6.2 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
380 |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
nucleus |
| Comments for localization |
bright signal |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |