| Gene name |
SPBC543.09 |
| Gene ID |
33/G06 |
| Gene synonyms/obsolete |
|
| Gene product |
respiratory chain
complex assembly protein; involved in postranslational
assembly and turnover of misfolded peptides; peptidase family
M41; AAA family ATPase; involved in mitochondrial signal
peptide processing |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2322 |
| ORF length (spliced) |
|
| Entry clone length |
2322 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
1878T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC543.09.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCGAAATCCCTTTTTGAC |
| Rev primer name |
SPBC543.09.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAGGCAGAAGGTTGTTGAGGA |
| Amino acid length |
773 |
| Molecular weight |
85.3 |
| Isoelectric point (calc.) |
9.9 |
| Signal SEQ |
|
| No. of transmembrane domain |
2 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LLSFAPTLLI |
| Localization (YFP) |
mitochondrion |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |