Gene name |
SPAC323.07c |
Gene ID |
34/H03 |
Gene synonyms/obsolete |
|
Gene product |
MatE family
transporter; unknown specificity; similar to Sp SPCC4B3.13 and
SPAC11D3.06 |
Entry clone |
Cloned |
ORF length (unspliced) |
1664 |
ORF length (spliced) |
1602 |
Entry clone length |
1664 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
613A:G / 1659G:A |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC323.07.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAAGCGTTTTTTCTCCAA |
Rev primer name |
SPAC323.07.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAACGTGGGTCAATCTATGA |
Amino acid length |
533 |
Molecular weight |
58.5 |
Isoelectric point (calc.) |
7.3 |
Signal SEQ |
|
No. of transmembrane domain |
10 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LLILAVLHI/LHFSFHGMLMI/LCSRVAYSLAL |
Localization (YFP) |
vacuole membrane |
Comments for localization |
aggregates by over
expression |
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |