Gene name |
SPCC962.05 |
Gene ID |
35/B08 |
Gene synonyms/obsolete |
|
Gene product |
hypothetical protein;
sequence orphan |
Entry clone |
Cloned |
ORF length (unspliced) |
1680 |
ORF length (spliced) |
1611 |
Entry clone length |
1680 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
221A:G / 928A:C /
1037C:T / 1079T:C / 1509A:T |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPCC962.05.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGGAGTACCTCGATTGGG |
Rev primer name |
SPCC962.05.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATGATTGATCTTTTTTAGTC |
Amino acid length |
536 |
Molecular weight |
61.8 |
Isoelectric point (calc.) |
6.8 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
526 |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
cytosol;
mitochondrion |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |