| Gene name |
SPBC1271.12 |
| Gene ID |
36/H03 |
| Gene synonyms/obsolete |
kes1 |
| Gene product |
oxysterol binding
protein; involved in ergosterol biosynthesis; similar to Sp
SPBC646.08c and SPBC354.07c and SPCC23B6.01; non-essential
|
| Entry clone |
Cloned |
| ORF length (unspliced) |
1848 |
| ORF length (spliced) |
1167 |
| Entry clone length |
1848 |
| No. of intron |
6 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC1271.12.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAGTAAAACAACGAGTTC |
| Rev primer name |
SPBC1271.12.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATCTAAGATAATACCAGAAA |
| Amino acid length |
388 |
| Molecular weight |
44.5 |
| Isoelectric point (calc.) |
7 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
periphery at site of
septum formation; ambiguous structure |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |