| Gene name |
SPAC227.15 |
| Gene ID |
38/F09 |
| Gene synonyms/obsolete |
|
| Gene product |
protein phosphatase
regulatory subunit |
| Entry clone |
Cloned in 2006
trial |
| ORF length (unspliced) |
2622 |
| ORF length (spliced) |
|
| Entry clone length |
2622 |
| No. of intron |
0 |
| Sequence status |
Partially
sequenced |
| Sequence results |
100% match in both
ends |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPAC227.15.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCTAACAAACCTGATGC |
| Rev primer name |
SPAC227.15.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATTCGCGAAATGATTTCAAA |
| Amino acid length |
873 |
| Molecular weight |
98.6 |
| Isoelectric point (calc.) |
5 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LDPHITPYLRL |
| Localization (YFP) |
cytosol; vacuole
membrane; cytoplasmic dots |
| Comments for localization |
|
| Effect of LMB on protein
localization |
changed to:
cytosol=nucleus; cytoplasmic dots; vacuole membrane |
| Microscope used for
observation |
Leica |