Gene name |
SPAC4G8.09 |
Gene ID |
38/F10 |
Gene synonyms/obsolete |
|
Gene product |
leucine-tRNA ligase;
involved in leucyl-tRNA aminoacylation; leucine-tRNA ligase
activity |
Entry clone |
Cloned |
ORF length (unspliced) |
2625 |
ORF length (spliced) |
|
Entry clone length |
2625 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
1158A:G /
1795A:G |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC4G8.09.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTTGAAATCCGTCGGTAC |
Rev primer name |
SPAC4G8.09.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAGTCGGTGACTAAACTGATG |
Amino acid length |
874 |
Molecular weight |
100.8 |
Isoelectric point (calc.) |
9.1 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
mitochondrion |
Comments for localization |
mitochondrion? |
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |