Gene name |
SPBC646.09c |
Gene ID |
40/F02 |
Gene synonyms/obsolete |
int6; yin6 |
Gene product |
eIF3 p48 subunit
eIF3/signalosome component; PCI domain; potential regulator
for microtubule function; involved in chromosome segregation;
chromosome segregation; interacts physically with Moe1p; froms
a complex with Moe1p; interacts physically with Scd1p;
interacts physically with Rpn5p; regulates the proteasome by
affecting its localization/assembly; cooperates with Ras1p to
mediate chromosome segregation; no apparent Sc ortholog;
deletion mutant results in inefficient separation of sister
chromatids; deletion mutant results in slow growth (at low
temperatures); disease associated, breast tumorigenesis;
functionally complemented by human INT6; deletion mutant is
proteosome defective |
Entry clone |
Cloned# |
ORF length (unspliced) |
1562 |
ORF length (spliced) |
1506 |
Entry clone length |
1562 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPBC646.09.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGGATCCGAGCTTAAGAG |
Rev primer name |
SPBC646.09.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAACAGTAGCATGCTTTAAC |
Amino acid length |
501 |
Molecular weight |
57.1 |
Isoelectric point (calc.) |
4.6 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LIFPLLEFLSL |
Localization (YFP) |
cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
changed to:
cytosol=nucleus |
Microscope used for
observation |
DeltaVision |