Gene name |
SPAC17A5.02c |
Gene ID |
40/F04 |
Gene synonyms/obsolete |
dbr1 |
Gene product |
RNA lariat debranching
enzyme; involved in mRNA splicing; involved in mRNA stability;
required for intronic snoRNA biosynthesis; functionally
complemented by human DRB1 |
Entry clone |
Cloned |
ORF length (unspliced) |
1530 |
ORF length (spliced) |
1401 |
Entry clone length |
1530 |
No. of intron |
3 |
Sequence status |
Finished |
Sequence results |
328A:G |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC17A5.02.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCGTACTTTGCAAGATT |
Rev primer name |
SPAC17A5.02.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATAGTACAATTTCGTTTGGA |
Amino acid length |
466 |
Molecular weight |
53.4 |
Isoelectric point (calc.) |
7.8 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nucleus;
mitochondrion |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |