| Gene name |
SPAC167.03c |
| Gene ID |
46/B10 |
| Gene synonyms/obsolete |
snu66 |
| Gene product |
coiled-coil protein
involved in mRNA splicing; putative U4/U6.U5 snRNP component;
predicted coiled-coil |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1950 |
| ORF length (spliced) |
|
| Entry clone length |
1950 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPAC167.03.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCTGGGAATTCAGGCGC |
| Rev primer name |
SPAC167.03.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAGAAAATTGGCTTGCGTTCT |
| Amino acid length |
649 |
| Molecular weight |
74.7 |
| Isoelectric point (calc.) |
4.8 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
542/68/288 |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
nucleus |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |