| Gene name |
SPCC757.04 |
| Gene ID |
46/F06 |
| Gene synonyms/obsolete |
|
| Gene product |
zinc finger protein;
zf-fungal Zn(2)-Cys(6) binuclear cluster domain |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2180 |
| ORF length (spliced) |
2055 |
| Entry clone length |
2180 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPCC757.04.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAGCCACATGGATAGTGA |
| Rev primer name |
SPCC757.04.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATAAGGTTTTTTCATTGAGC |
| Amino acid length |
684 |
| Molecular weight |
78.4 |
| Isoelectric point (calc.) |
5.1 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LTLIRLDLSL/LTSVQCLLCL |
| Localization (YFP) |
cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
changed to:
nucleus>>cytosol |
| Microscope used for
observation |
Leica |