Gene name |
SPAC22H12.05c |
Gene ID |
46/F07 |
Gene synonyms/obsolete |
|
Gene product |
fasciclin domain
protein (3); cell-adhesion molecule in higher eukaryotes |
Entry clone |
Cloned |
ORF length (unspliced) |
2187 |
ORF length (spliced) |
|
Entry clone length |
2187 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPAC22H12.05.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAACCTTCAATTTCGGTT |
Rev primer name |
SPAC22H12.05.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATACGGATAAAGACGAAGAA |
Amino acid length |
728 |
Molecular weight |
82.5 |
Isoelectric point (calc.) |
5.7 |
Signal SEQ |
Predicted
(N-terminus) |
No. of transmembrane domain |
1 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LQFRLYLLFI |
Localization (YFP) |
vacuole membrane |
Comments for localization |
aggregates by over
expression |
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Confocal,
DeltaVision |