| Gene name |
SPBC947.11c |
| Gene ID |
47/C12 |
| Gene synonyms/obsolete |
|
| Gene product |
Sc EFD1 is required
for stability of direct DNA repeats; AAA family ATPase
(inferred from context) |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2854 |
| ORF length (spliced) |
2763 |
| Entry clone length |
2854 |
| No. of intron |
2 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPBC947.11.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCAAATTGTAGGCTATTT |
| Rev primer name |
SPBC947.11.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAATCTAGAGTTGTTGAAAAC |
| Amino acid length |
920 |
| Molecular weight |
103.8 |
| Isoelectric point (calc.) |
5.2 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
cytosol=nucleus |
| Comments for localization |
except nucleolus |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |