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Schizosaccharomyces pombe Postgenome Database

Gene name SPAPB24D3.01
Gene ID 49/C04
Gene synonyms/obsolete SPAPB2C8.02
Gene product involved in transcriptional regulation; zinc finger protein; zf-fungal Zn(2)-Cys(6) binuclear cluster domain
Entry clone Cloned
ORF length (unspliced) 1785
ORF length (spliced)
Entry clone length 1785
No. of intron 0
Sequence status Finished
Sequence results 775T:C / 913T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAP24D3.01.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGACTTCTGTGGAAAAGGC
Rev primer name SPAP24D3.01.Rv
Rev primer SEQ AGAAAGCTGGGTACGTCAAAAAGCTCGCAAAA
Amino acid length 594
Molecular weight 68.9
Isoelectric point (calc.) 7
Signal SEQ
No. of transmembrane domain 1
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LVSFYSVLAL/LEKIQALLI/LYCPFTPFLVL
Localization (YFP) nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Confocal

Image information
YFP 2 images) See all images

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