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Schizosaccharomyces pombe Postgenome Database

Gene name SPBP19A11.02c
Gene ID 50/A11
Gene synonyms/obsolete
Gene product hypothetical protein; possibly Sp specific; GPI anchored protein (pers. comm. Birgit Eisenhaber); glycoprotein; no apparent Sc ortholog
Entry clone Cloned directly into pDUAL-FFH1
ORF length (unspliced) 735
ORF length (spliced)
Entry clone length 735
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPBP19A11.02.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCAGTTCTCTGTCCTTTG
Rev primer name SPBP19A11.02.Rv
Rev primer SEQ AGAAAGCTGGGTAAGCCAAAAAGGCGAAGGCA
Amino acid length 244
Molecular weight 25.3
Isoelectric point (calc.) 6.7
Signal SEQ Predicted (N-terminus)
No. of transmembrane domain 1
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) ER
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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