Gene name |
SPBP19A11.02c |
Gene ID |
50/A11 |
Gene synonyms/obsolete |
|
Gene product |
hypothetical protein;
possibly Sp specific; GPI anchored protein (pers. comm. Birgit
Eisenhaber); glycoprotein; no apparent Sc ortholog |
Entry clone |
Cloned directly into
pDUAL-FFH1 |
ORF length (unspliced) |
735 |
ORF length (spliced) |
|
Entry clone length |
735 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPBP19A11.02.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCAGTTCTCTGTCCTTTG |
Rev primer name |
SPBP19A11.02.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAGCCAAAAAGGCGAAGGCA |
Amino acid length |
244 |
Molecular weight |
25.3 |
Isoelectric point (calc.) |
6.7 |
Signal SEQ |
Predicted
(N-terminus) |
No. of transmembrane domain |
1 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
ER |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |