| Gene name |
SPBP18G5.01 |
| Gene ID |
50/B01 |
| Gene synonyms/obsolete |
SPBC36B7.09 |
| Gene product |
possibly involved in
serine/threonine protein kinase; eukaryotic initiation factor
2 alpha (eIF2-alpha) kinase; involved in amino acid control
response |
| Entry clone |
Cloned directly into
pDUAL-FFH1 |
| ORF length (unspliced) |
4780 |
| ORF length (spliced) |
4731 |
| Entry clone length |
4780 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPBP18G5.01.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGATGCGGCGAAACGTCT |
| Rev primer name |
SPBP18G5.01.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATTTAAGTCCATAAAGATAA |
| Amino acid length |
1576 |
| Molecular weight |
181.1 |
| Isoelectric point (calc.) |
6.2 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
487 |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LVTFNRVLPL/LFSLLQTVLTL/LAELHRLGI/LNKFSSLKL |
| Localization (YFP) |
no expression
clone |
| Comments for localization |
|
| Effect of LMB on protein
localization |
not determined |
| Microscope used for
observation |
|