Gene name |
SPAPB18E9.05c |
Gene ID |
50/B02 |
Gene synonyms/obsolete |
|
Gene product |
hypothetical protein;
sequence orphan |
Entry clone |
Cloned |
ORF length (unspliced) |
273 |
ORF length (spliced) |
|
Entry clone length |
273 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
59T:C |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAPB18E9.05.Fd |
Fwd primer SEQ |
GGGGACAAGTTTGTACAAAAAAGCAGGCTCTCATATGAATGAAGTAATCAGTCA |
Rev primer name |
SPAPB18E9.05.Rv |
Rev primer SEQ |
GGGGACCACTTTGTACAAGAAAGCTGGGTATATATAGGAGAAATAAATG |
Amino acid length |
90 |
Molecular weight |
10.3 |
Isoelectric point (calc.) |
8.2 |
Signal SEQ |
Predicted
(N-terminus) |
No. of transmembrane domain |
2 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
ER |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |