Gene name |
SPAC227.01c |
Gene ID |
50/C11 |
Gene synonyms/obsolete |
SPAPB21F2.04c |
Gene product |
ER lumen protein
retaining receptor activity; involved in protein-ER retention;
GPI anchored protein |
Entry clone |
Cloned |
ORF length (unspliced) |
1122 |
ORF length (spliced) |
|
Entry clone length |
1122 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
209T:C |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAPB17E12.10.Fd |
Fwd primer SEQ |
GGGGACAAGTTTGTACAAAAAAGCAGGCTCTCATATGTCTATGAGAATTTGGAG |
Rev primer name |
SPAPB17E12.10.Rv |
Rev primer SEQ |
GGGGACCACTTTGTACAAGAAAGCTGGGTAAATGTTCAAAAGCTTAGAA |
Amino acid length |
373 |
Molecular weight |
43.6 |
Isoelectric point (calc.) |
9.1 |
Signal SEQ |
Predicted
(N-terminus) |
No. of transmembrane domain |
4 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
vacuole membrane;
cytoplasmic dots |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |