| Gene name |
SPBC11B10.03 |
| Gene ID |
50/F05 |
| Gene synonyms/obsolete |
pi008;
SPACTOKYO_453.28c |
| Gene product |
Golgi transport
complex subunit; conserved eukaryotic protein |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1372 |
| ORF length (spliced) |
1203 |
| Entry clone length |
1372 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
20C:T / 551G:A /
1102T:C / 1346T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC11B10.03.Fd |
| Fwd primer SEQ |
GGGGACAAGTTTGTACAAAAAAGCAGGCTCTCATATGAAGATGCAAGTTTCTAC |
| Rev primer name |
SPBC11B10.03.Rv |
| Rev primer SEQ |
GGGGACCACTTTGTACAAGAAAGCTGGGTACGCCTGTTTCTCAGAAGAT |
| Amino acid length |
400 |
| Molecular weight |
46.7 |
| Isoelectric point (calc.) |
8.3 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LVVKLTEKLIL/LSSFVRKLQL |
| Localization (YFP) |
no apparent
signal |
| Comments for localization |
|
| Effect of LMB on protein
localization |
not determined |
| Microscope used for
observation |
DeltaVision |