| Gene name |
SPAC750.02c |
| Gene ID |
51/D10 |
| Gene synonyms/obsolete |
|
| Gene product |
putative membrane
transporter; telomeric duplication; similar to Sp
SPBC1348.05 |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1458 |
| ORF length (spliced) |
|
| Entry clone length |
1458 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
1287A:T |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPAC750.02.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAAAGAAGAGTCAATTTT |
| Rev primer name |
SPAC750.02.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAGTATTTTGAGGGGTCAAAT |
| Amino acid length |
485 |
| Molecular weight |
54 |
| Isoelectric point (calc.) |
9.7 |
| Signal SEQ |
|
| No. of transmembrane domain |
10 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LGATLYTLGI/LMYISITLGL |
| Localization (YFP) |
Golgi; periphery,
especially at cell tip and site of septum formation |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |