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September 11, 2012

Screening technique uncovers five new plant activator compounds

Immune-priming compounds identified using new screening technique protect crops without affecting plant growth, crop yield

A new high-throughput screening technique developed by researchers at the RIKEN Plant Science Center (PSC) has been used to uncover five novel immune-priming compounds in Arabidopsis plants. Discovery of the compounds, which enhance disease resistance without impacting plant growth or crop yield, establishes the new technique as a powerful asset in the battle to protect crops from damaging pathogens.

Crop protection by plant activators

Crop protection by plant activators

Plant activators, compounds that activate a plant's immune system in response to invasion by pathogens, play a crucial role in crop survival by triggering a range of immune responses. Unlike pesticides, plant activators are not pathogen specific and also not affected by drug resistance, making them ideal for use in agriculture. Wet-rice farmers across East Asia use plant activators as a sustainable means to enhance crop durability without the environmental consequences of microbial pesticides.

One of the key problems of plant activators, however, is that the activation of plant responses they trigger is often associated with arrested growth and reductions in crop yield. Determining why this is so is difficult because despite their widespread use, the molecular mechanisms governing how plant activators work are largely unknown.

The new screening technique developed by the PSC team overcomes this challenge by distinguishing between compounds that induce immune responses on their own from those that do so exclusively in the presence of a pathogen. The former class of compounds, which includes known plant activators, can be toxic to cells and was thus eliminated in the screening. The resulting five compounds, identified from a total of 10,000 compounds screened, enhance resistance against pathogenic Pseudomonas bacteria by priming immune response without directly activating defense genes.

Further investigation revealed that the five compounds inhibit two enzymes that inactivate the defense hormone salicylic acid (SA glucosyltransferases or SAGTs), and gene knockout experiments confirmed that plants without these enzymes exhibit enhanced disease resistance. Together, the results establish the effectiveness of the new technique for finding useful plant activators and the power of SA metabolism as a strategy for crop protection.

Reference

  • Yoshiteru Noutoshi, Masateru Okazaki, Tatsuya Kida, Yuta Nishina, Yoshihiko Morishita, Takumi Ogawa, Hideyuki Suzuki, Daisuke Shibata, Yusuke Jikumaru, Atsushi Hanada, Yuji Kamiya and Ken Shirasu. “Novel plant immune-priming compounds identified via high-throughput chemical screening target salicylic-acid glucosyltransferases in Arabidopsis”. The Plant Cell (2012). doi:10.1105/tpc.112.098343

Contact

Ken Shirasu
Plant Immunity Research Group
RIKEN Plant Science Center

Jens Wilkinson
RIKEN Global Relations and Research Coordination Office
Tel: +81-(0)48-462-1225 / Fax: +81-(0)48-463-3687
Email: pr@riken.jp

Figure 1: Crop protection methods and their targets.

Figure 2: Original screening method for immune response regulators.

Figure 3: Molecular structure of Imprimatins

Figure 4: Original screening method for immune response modulators.

Figure 5: Plant immunity signaling and the action point of Imprimatins.