Centers & Labs

RIKEN Center for Brain Science

Laboratory for Protein Conformation Diseases

Laboratory Head: Motomasa Tanaka (Ph.D.)
Motomasa  Tanaka(Ph.D.)

We are investigating the molecular basis of neuropsychiatric diseases using both in vivo and in vitro systems with a variety of techniques including neuroscience, genetics, chemical biology and structural biology. In conjunction, we are seeking to develop novel proteomics or genomics technologies to advance our understanding of the mechanisms of cellular aging and abnormal mRNA translation. Furthermore, we will uncover the structural basis of formation, disaggregation and propagation of amyloid fibers using the yeast prion system that we have established.

Main Research Field

Biological Sciences

Related Research Fields

Medicine, dentistry, and pharmacy

Keywords

  • Neuropsychiatric disease
  • Protein aggregation
  • Amyloid
  • Nascent chain biology

Selected Publications

  1. Sugiyama, S., Tanaka, M.:
    "Distinct segregation patterns of yeast cell-peripheral proteins uncovered by a method for protein segregatome analysis."
    Proc Natl Acad Sci U. S. A., 116, 8909-8918 (2019).
  2. Hui, K.K., Takashima, N., Watanabe, A., Chater, T.E., Matsukawa, H., Nekooki-Machida, Y., Nilsson, P., Endo, R., Goda, Y., Saido, T.C., Yoshikawa, T., Tanaka., M.:
    "GABARAPs dysfunction by autophagy deficiency in adolescent brain impairs GABAA receptor trafficking and social behavior."
    Science Advances, 5(4), eaau8237 (2019).
  3. Endo R., Takashima N., Nekooki-Machida Y., Komi Y., Hui K.K., Takao M., Akatsu H., Murayama S., Sawa A., and Tanaka M.:
    "TDP-43 and DISC1 Co-Aggregation Disrupts Dendritic Local Translation and Mental Function in FTLD."
    Biol.Psychiatry, 84, 509-521 (2018).
  4. Chen C.W., Tanaka M.:
    "Genome-Wide Translation Profiling by Ribosome-Bound tRNA Capture."
    Cell Rep., 23, 608-621 (2018).
  5. Ohhashi Y., Yamaguchi Y., Kurahashi H., Kamatari Y.O., Sugiyama S., Uluca B., Piechatzek T., Komi Y., Shida T., Müller H., Hanashima S., Heise H., Kuwata K, and Tanaka M.:
    "Molecular basis for diversification of yeast prion strain conformation."
    Proc. Natl. Acad. Sci. U. S. A., 115, 2389-2394 (2018).
  6. Tanaka, M., Ishizuka, K., Nekooki-Machida, Y., Endo, R., Takashima, N., Sasaki, H., Komi, Y., Gathercole, A., Huston, E., Ishii, K., Hui, K.K., Kurosawa, M., Kim, S.H., Nukina, N.,Takimoto, E., Houslay, M.D., and Sawa, A.:
    "Aggregation of scaffolding protein DISC1 dysregulates phosphodiesterase 4 in Huntington's disease."
    J. Clin. Invest., 127, 1438-1450 (2017).
  7. Suzuki, G., Weissman, J.S., and Tanaka, M.:
    "[KIL-d] protein element confers antiviral activity via catastrophic viral mutagenesis."
    Mol. Cell, 60, 651-660 (2015).
  8. Tanaka, M. and Komi, Y.:
    "Layers of structure and function in protein aggregation."
    Nat. Chem. Biol., 11, 373-377 (2015).
  9. Suzuki, G., Shimazu, N., and Tanaka, M.:
    "A Yeast Prion, Mod 5, Promotes Acquired Drug Resistance and Cell Survival Under Environmental Stress."
    Science 336, 355-359 (2012).
  10. Ohhashi, Y., Ito, K., Toyama, B.H., Weissman, J.S., and Tanaka, M.:
    "Differences in prion strain conformations result from non-native interactions in a nucleus."
    Nat. Chem. Biol. 6, 225-230 (2010).

Lab Members

Principal Investigator

Motomasa Tanaka
Laboratory Head

Core Members

Yusuke Komi
Research Scientist
Ryo Endo
Research Scientist
Kai-Wan Kelvin Hui
Research Scientist
Marek Konrad Krzyzanowski
Research Scientist
Yi-Kai Chen
Visiting Researcher
Yoshiko Nakagawa
Junior Research Associate
Chih-Hao Shen
International Program Associate
Noriko Takashima
Technical Staff I
John William Burke
Technical Staff II