Centers & Labs

Chief Scientist Laboratories

RNA Systems Biochemistry Laboratory

Chief Scientist: Shintaro Iwasaki (Ph.D.)
Shintaro  Iwasaki(Ph.D.)

“The central dogma of molecular biology”, which represents information flow from DNA to RNA to protein, has been most basic principle in life. Recent quantitative and comprehensive analysis revealed that amount of RNA could not simply correlate with protein abundance in cells, suggesting that “translation control” significantly contributes to gene expression in more general than as we previously expected. Our laboratory tackles the unknown mechanism of translation control, by the combination of next-generation deep sequencing and classical biochemistry.

Main Research Field

Biology

Related Research Fields

Biological Sciences

Life science / Genome science

Keywords

  • translation
  • RNA
  • Inhibitors

Selected Publications

  1. Padron A., Iwasaki S., and Ingolia NT.:
    "Proximity RNA labeling by APEX-Seq reveals the organization of translation initiation complexes and repressive RNA granules"
    Mol. Cell. 75(4):875-887. (2019)
  2. Iwasaki S., Iwasaki W., Takahashi M. (equal contribution), Sakamoto A., Watanabe C., Shichino Y., Floor SN., Fujiwara K., Mito M., Dodo K., Sodeoka M., Imataka H., Honma T., Fukuzawa K., Ito T., and Ingolia NT.:
    "The translation inhibitor Rocaglamide targets a bimolecular cavity between eIF4A and polypurine RNA"
    Mol. Cell. 73(4):738-748. (2019)
  3. AkichikaS., Hirano S (equal contribution)., Shichino Y., Suzuki T., Nishimasu H., Ishitani R., Sugita A., Hirose Y., Iwasaki S., Nureki O., and Suzuki T.:
    "Cap-specific terminal N6-methylation of RNA by an RNA polymerase II?associated methyltransferase"
    Science. 11;363(6423) (2019)
  4. Kurihara Y., Makita Y., Kawashima M., Fujita T., Iwasaki S., and Matsui M.:
    "Transcripts from downstream alternative transcription start sites evade uORF-mediated inhibition of gene expression in Arabidopsis
    Proc Natl Acad Sci U S A. 115(30):7831-7836 (2018)
  5. Iwasaki S. and Ingolia NT.:
    "The Growing Toolbox for Protein Synthesis Studies"
    Trends Biochem Sci. 42(8):612-624 (2017)
  6. Iwasaki S., Floor SN., and Ingolia NT.:
    "Rocaglates convert DEAD-box protein eIF4A into a sequence-selective translational repressor"
    Nature 534(7608), 558-61 (2016)
  7. Werner A., Iwasaki S., Teerikorpi N., Fedrigo I., Ingolia NT., and Rape M.:
    "Cell fate determination by ubiquitin-dependent regulation of ribosome function"
    Nature 525(7570), 523-7 (2015)
  8. Iwasaki S., Sasaki MH. (equal contribution), Sakaguchi Y., Suzuki T., Tadakuma H., and Tomari Y.:
    "Defining fundamental steps in the assembly of Drosophila RNAi enzyme comple"
    Nature 521(7553), 533-6 (2015)
  9. Iwasaki S., Kobayashi M. (equal contribution), Yoda M., Sakaguchi Y., Katsuma S., Suzuki T., and Tomari Y.:
    "Hsc70/Hsp90 chaperone machinery mediates ATP-dependent RISC loading of small RNA duplexes"
    Mol. Cell. 39(2), 292-9 (2010)
  10. Iwasaki S., Kawamata T., and Tomari Y.:
    "Drosophila Argonaute1 and Argonaute2 employ distinct mechanisms for translational repression"
    Mol Cell. 34(1), 58-67 (2009)

Lab Members

Principal Investigator

Shintaro Iwasaki
Chief Scientist

Core Members

Eriko Matsuura
Research Scientist
Yuichi Shichino
Visiting Researcher
Yusuke Kimura
Junior Research Associate
Mingming Chen
International Program Associate
Tomoya Fujita
Student Trainee
Peixun Han
Student Trainee
Hironori Saito
Student Trainee
Shiho Makino
Research Fellow
Mari Mito
Technical Staff I
Rie Yokoyama
Assistant

Contact Information

S205 Bioscience Bldg,
2-1 Hirosawa,
Wako, Saitama
351-0198, Japan

Email: shintaro.iwasaki [at] riken.jp

Related Links

Recent Research Results

Annual research report