Centers & Labs

Chief Scientist Laboratories

RNA Systems Biochemistry Laboratory

Chief Scientist: Shintaro Iwasaki (Ph.D.)
Shintaro  Iwasaki(Ph.D.)

“The central dogma of molecular biology”, which represents information flow from DNA to RNA to protein, has been most basic principle in life. Recent quantitative and comprehensive analysis revealed that amount of RNA could not simply correlate with protein abundance in cells, suggesting that “translation control” significantly contributes to gene expression in more general than as we previously expected. Our laboratory tackles the unknown mechanism of translation control, by the combination of next-generation deep sequencing and classical biochemistry.

Main Research Field

Biology

Related Research Fields

Biological Sciences

Life science / Genome science

Keywords

  • translation
  • RNA
  • Inhibitors

Selected Publications

  1. Matsuo Y., Ikeuchi K., Saeki Y., Iwasaki S., Schmidt C., Udagawa T., Sato F., Tsuchiya H., Becker T., Tanaka K., Ingolia NT., Beckmann R., & Inada T.:
    "Ubiquitination of stalled ribosome triggers ribosome-associated quality control"
    Nat Commun. 8(1):159. (2017)
  2. Iwasaki S., Ingolia NT.:
    "The Growing Toolbox for Protein Synthesis Studies"
    Trends Biochem Sci. pii: S0968-0004(17)30092-0. (2017)
  3. Ishikawa K., Makanae K., Iwasaki S., Ingolia NT., Moriya H.:
    "Post-Translational Dosage Compensation Buffers Genetic Perturbations to Stoichiometry of Protein Complexes."
    PLoS Genet. 25;13(1):e1006554. (2017)
  4. Iwasaki S., Floor SN., and Ingolia NT.:
    "Rocaglates convert DEAD-box protein eIF4A into a sequence-selective translational repressor"
    Nature 15, 534(7608), 558-61 (2016)
  5. Werner A., Iwasaki S., Teerikorpi N., Fedrigo I., Ingolia NT., and Rape M.:
    "Cell fate determination by ubiquitin-dependent regulation of ribosome function"
    Nature 24, 525(7570), 523-7 (2015)
  6. Iwasaki S.*, Sasaki MH.*, Sakaguchi Y., Suzuki T., Tadakuma H., and Tomari Y. (* equal contribution):
    "Defining fundamental steps in the assembly of Drosophila RNAi enzyme complex"
    Nature 28, 521(7553), 533-6 (2015)
  7. Iwasaki S.*, Kobayashi M.*, Yoda M., Sakaguchi Y., Katsuma S., Suzuki T., and Tomari Y. (* equal contribution):
    "Hsc70/Hsp90 chaperone machinery mediates ATP-dependent RISC loading of small RNA duplexes"
    Mol. Cell 30, 39(2), 292-9 (2010)
  8. Yoda M., Kawamata T., Paroo Z., Ye X., Iwasaki S., Liu Q., and Tomari Y.:
    "ATP-dependent human RISC assembly pathways"
    Nat. Struct. Mol. Biol. 17(1), 17-23 (2010)
  9. Iwasaki S., Kawamata T., and Tomari Y.:
    "Drosophila Argonaute1 and Argonaute2 employ distinct mechanisms for translational repression"
    Mol Cell. 10, 34(1), 58-67 (2009)
  10. Takeda A., Iwasaki S., Watanabe T., Utsumi M., and Watanabe Y.:
    "The mechanism selecting the guide strand from small RNA duplexes is different among Argonaute proteins"
    Plant Cell Physiol. 49(4), 493-500 (2008)

Lab Members

Principal Investigator

Shintaro Iwasaki
Chief Scientist

Core Members

Yuichi Shichino
Postdoctoral Researcher
Mari Mito
Technical Staff I
Shiho Makino
Research Fellow
Yusuke Kimura
Student Trainee
Tomoya Fujita
Student Trainee
Rie Yokoyama
Assistant

Contact information

S205 Bioscience Bldg,
2-1 Hirosawa,
Wako, Saitama
351-0198, Japan

Email: shintaro.iwasaki [at] riken.jp

Related links

Annual research report