Centers & Labs

RIKEN Center for Sustainable Resource Science

Cell Factory Research Team

Team Leader: Akihiko Kondo (Ph.D.)
Akihiko  Kondo(Ph.D.)

The Cell Factory Research Team organized in fiscal year 2012 develops optimum technologies for the construction of microorganisms producing biomonomers, a material in chemical products, from plant materials. We have also reinforced our collaboration with the industrial world in order to develop technologies to produce those biomonomers from plant materials such as herbaceous and woody biomasses.

Main Research Field


Related Research Fields

Chemistry / Biology


  • bioproduction
  • biorefinery
  • Synthetic bioengineering

Selected Publications

  1. Shirai T, Osanai T, Kondo A.
    "Designing intracellular metabolism for production of target compounds by introducing a heterologous metabolic reaction based on a Synechosystis sp. 6803 genome-scale model."
    Microb Cell Fact. 2016 Jan 18;15:13. doi: 10.1186/s12934-016-0416-8.
  2. Noda S, Shirai T, Oyama S, Kondo A.:
    "Metabolic design of a platform Escherichia coli strain producing various chorismate derivatives."
    Metab Eng. 33:119-29. doi: 10.1016/j.ymben.2015.11.007. (2016)
  3. Osanai T, Shirai T, Iijima H, Nakaya Y, Okamoto M, Kondo A, Hirai MY.:
    "Genetic manipulation of a metabolic enzyme and a transcriptional regulator increasing succinate excretion from unicellular cyanobacterium."
    Front Microbiol. 6, doi: 10.3389/fmicb.2015.01064. (2015)
  4. Iijima H, Shirai T, Okamoto M, Kondo A, Hirai MY, Osanai T.:
    "Changes in primary metabolism under light and dark conditions in response to overproduction of a response regulator RpaA in the unicellular cyanobacterium Synechocystis sp. PCC 6803."
    Front Microbiol. 6, doi: 10.3389/fmicb.2015.00888. (2015)
  5. Sasaki K, Okamoto M, Shirai T, Tsuge Y, Teramura H, Sasaki D, Kawaguchi H, Hasunuma T, Ogino C, Matsuda F, Kikuchi J, Kondo A.:
    "Precipitate obtained following membrane separation of hydrothermally pretreated rice straw liquid revealed by 2D NMR to have high lignin content."
    Biotechnol Biofuels. 8 doi: 10.1186/s13068-015-0273-4. (2015)
  6. Noda S, Shirai T, Mochida K, Matsuda F, Oyama S, Okamoto M, Kondo A.:
    "Evaluation of Brachypodium distachyon L-Tyrosine Decarboxylase Using L-Tyrosine Over-Producing Saccharomyces cerevisiae."
    PLoS One. 21, e0125488 (2015).
  7. Noda, S., Kawai, Y., Tanaka, T., Kondo, A.:
    "4-Vinylphenol biosynthesis from cellulose as the sole carbon source using phenolic acid decarboxylase-and tyrosine ammonia lyase-expressing Streptomyces lividans."
    Bioresour Technol, 180, 59-65. (2015)
  8. Noda, S., Matsumoto, T., Tanaka, T., Kondo, A.:
    "Secretory production of tetrameric native full-length streptavidin with thermostability using Streptomyces lividans as a host."
    Microb Cell Fact, 14, 5 (2015)
  9. Osanai T, Shirai T, Iijima H, Kuwahara A, Suzuki I, Kondo A, Hirai MY.:
    "Alteration of cyanobacterial sugar and amino acid metabolism by overexpression hik8, encoding a KaiC-associated histidine kinase."
    Environ Microbiol. 17, 2430-2340. (2015)
  10. Shirai T, Matsuda F, Okamoto M, Kondo A.:
    "Evaluation of control mechanisms for Saccharomyces cerevisiae central metabolic reactions using metabolome data of eight single-gene deletion mutants."
    Appl Microbiol Biotechnol. 97, 3569-3577. (2013)

Lab Members

Principal Investigator

Akihiko Kondo
Team Leader

Core Members

Tomokazu Shirai
Deputy Team Leader
Shuhei Noda
Research Scientist
Yutaro Mori
Postdoctoral Researcher
Sachiko Ooyama
Technical Staff II
Ryoko Orishimo
Technical Staff II