Centers & Labs

RIKEN Center for Sustainable Resource Science

Mass Spectrometry and Microscopy Unit

Unit Leader: Kazuki Saito (Ph.D.)
Kazuki  Saito(Ph.D.)

Mass spectrometric and microscopic analyses are fundamental analytical technology in plant science and sustainable resource science. Our unit develops and executes the analyses based on mass spectrometry for the study of plant metabolome and hormonome and on microscopy for the ultrastructural observation of the plant cells.

Main Research Field

Biological Sciences

Related Research Fields

Chemistry / Biology / Agricultural sciences / Medicine, dentistry, and pharmacy

Keywords

  • metabolomics
  • phytohormone
  • mass spectrometry
  • microscopy

Selected Publications

  1. Nakabayashi, R., Hashimoto, K., Toyooka, K. and Saito, K.:
    "Top-down Metabolomic Approaches for Nitrogen-Containing Metabolites"
    Analytical Chemistry, 89, 2698-2703 (2017)
  2. Sawada, Y., Tsukaya, H., Li, Y., Sato, M., Kawade, K. and Hirai, M.Y.:
    "A Novel Method for Single-grain-based Metabolic Profiling of Arabidopsis Seed"
    Metabolomics, 13, 75 (2017)
  3. Toyooka, K.:
    "Development of correlative light and electron microscopy to observe green fluorescent protein-labeled organelles embedded in resin using field-emission electron scanning microscope"
    Plant Morphology, 28, 15-21 (2016)
  4. Toyooka, K., Sato, M., Kutsuna, N., Higaki, T., Sawaki, F., Wakazaki, M., Goto, Y., Hasezawa, S., Nagata, N. and Matsuoka, K.:
    "Wide-range high-resolution transmission electron microscopy reveals morphological and distributional changes of endomembrane compartments during log-to-stationary transition of growth phase in tobacco BY-2 cells"
    Plant Cell Physiology, 55, 1544-1555 (2014)
  5. Nakabayashi, R., Sawada, Y., Yamada, Y., Suzuki, M., Hirai, M.Y., Sakurai, T and Saito, K.:
    "Combination of Liquid Chromatography-Fourier Transform-Ion Cyclotron Resonance-Mass Spectrometry with 13C-labeling for Chemical Assignment of Sulfur-containing Metabolites in Onion Bulbs"
    Analytical Chemistry, 85, 1310–1315 (2013)
  6. Sawada, Y. and Hirai, M.Y.:
    "Integrated LC-MS/MS system for plant metabolomics"
    Computational and Structural Biotechnology Journal, 4, e20131011. (2013)
  7. Kojima, M., Kamada-Nobusada, T., Komatsu, H., Takei, K., Kuroha, T., Mizutani, M., Ashikari, M., Ueguchi-Tanaka, M., Matsuoka, M., Suzuki, K. and Sakakibara, H.:
    "Highly-sensitive and High-throughput analysis of plant hormones using MS-probe modification and liquid chromatography-tandem mass spectrometry: an application for hormone profiling in Oryza sativa"
    Plant Cell Physiology, 50, 1201-1214 (2009)

Lab Members

Principal Investigator

Kazuki Saito
Unit Leader

Core Members

Hitoshi Sakakibara
Deputy Unit Leader
Masami Hirai
Deputy Unit Leader
Kiminori Toyooka
Senior Research Scientist
Yuji Sawada
Research Scientist
Ryo Nakabayashi
Research Scientist
Mikiko Kojima
Technical Scientist
Mayuko Sato
Technical Scientist
Makoto Kobayashi
Technical Staff I
Atsushi Kurotani
Technical Staff I
Tetsuya Mori
Technical Staff I
Yumiko Takebayashi
Technical Staff I
Muneo Sato
Technical Staff I
Ryosuke Sasaki
Technical Staff I
Ayako Miya
Technical Staff I
Mayumi Wakazaki
Technical Staff II
Yutaka Yamada
Technical Staff II
Kei Hashimoto
Technical Staff II